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PRODUCT DESCRIPTION:
AquaTru ® Nitrite Test Kit
Kordon's AquaTru Test Kits were designed for accuracy with several important criteria in mind:
1-Dry,stable reagents
2-Dated reagents
3-Accurate, translucent color comparators
4-Comprehensive instructions
5-Measurement ranges that are correct for aquarium use.
PRODUCT DESCRIPTION:
AquaTru Water Quality Test Kits pioneered the use of dry reagents for use in aquarium water testing. The major advantage of dry reagents is in their shelf life. Dry reagents are more stable than liquid reagents, which means the aquarist can perform tests with the confidence of knowing the results will be as accurate as possible when using a "colorimetric" type test. Kordon also dates their reagents. All manufacturer's reagents, whether dry or liquid, are subject to degradation. Because the reagent chemicals break down over time, it is important to know the age of the reagents and their expected life.
Made in U.S.A.
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GENERAL INFORMATION
Nitrite is an intermediate compound formed in the process of oxidation of nitrogen containing compounds to less toxic nitrate. It is formed as a product of ammonia metabolism by a type of autotrophic nitrifying bacteria and is normally converted to nitrate by a second type of autotrophic nitrifying bacteria. Nitrite, like un-ionized ammonia (NH 3) , is toxic to fishes at very low concentrations and therefore should be carefully monitored. Toxic levels of nitrite are most likely found when the nitrifying bacteria have not been well established in the aquarium filter bed (as in the case of a new aquarium set-up). Nitrifying bacteria, if present, will convert ammonia to nitrite which will quickly rise to toxic and eventually lethal levels since the bacteria are not yet able to oxidize the nitrite to the less toxic nitrate. In a typical newly set up aquarium, the nitrite levels will begin to rise in about 7 to 8 days, peak in approximately 15 days, and decline to undetectable levels in about 30 days. Nitrite levels as low as 0.2 ppm can cause stress to many aquarium fishes. The Kordon AquaTru Nitrite Test Kit provides a simple and accurate means of monitoring levels of nitrite in solution. Once nitrifying bacteria are established and remain undisturbed, high levels of nitrite should not occur.
AQUA-TRU ADVANTAGES
- Fast and accurate readings appropriate to fish health. Graduations of concentration are very small to facilitate accurate readings of highly toxic levels of nitrite.
- Highly stable powdered reagents. Sealed in foil pillows and dated to insure freshness.
- Easy-to-use kit: simple step-by-step instructions and explanations.
- Practical expression of Nitrite concentration: readings of nitrite are expressed both as nitrite ion and as nitrite nitrogen.
CHEMISTRY OF COLOR REACTIONS
Nitrite reacts with two acids in the Nitrite Powder Pillow Reagent (sulfanilic and then chromotropic) to form a red complex whose concentration is directly proportional to the amount of nitrite originally present. By measuring the intensity of the reddish complex with the color comparator, the concentration of the nitrite is easily determined.
INTERFERENCES
There are certain compounds (chlorine, nitrogen trichloride) which can interfere with the color reaction of nitrite. However, their presence in aquarium water in quantities that would significantly alter the reading is highly unlikely. For this reason, no special precautions are necessary to prevent such interference.
GUIDELINES FOR ACCURATE TEST RESULTS
- Rinse the color cube 2-3 times before collecting samples so that residual chemicals from any previous tests do not interfere with results. It is suggested that tap water be used for the initial rinse so chemicals do not get into the aquarium when the cube is dipped. Aquarium water should be used for final rinses so that the residual rinse water will not affect the readings. Discard the rinse water each time so that residual chemicals are not poured into the aquarium.
- When collecting water samples, take care to obtain a representative sample. For example, temperature differences near the surface can result in pH readings that differ from midwater samples. Although the affect is minimal, sampling techniques should be tested, Take two samples and analyze according to kit instructions; any variation in the results indicates that more care should be exercised when collecting samples. A recommended method is to (a) submerge the rinsed, capped cube to midwater, (b) open the cube and allow to fill, (c) cap while still submerged and remove, and (d) drain water to correct level (discard excess).
- Test the water sample immediately following collection. Time elapsed between collection and analysis may affect test results.
- When matching the test solution to the color cube, use white paper as a background and read into normal daylight or incandescent light sources.
DIRECTIONS
- Following the "Guidelines for accurate test results", obtain a representative water sample for testing.
- Open one Powder Pillow Reagent (marked LR NITRITE). Add the the contents to the water sample, cap, and mix for one minute.
- Wait 10 minutes to allow for complete color development and determine the nitrite concentration by matching the solution color to the closest shade on the color comparator. After use, discard the contents of the cube and rinse with tap water. Do not return any of the water to the aquarium.
- The units of the reading obtained is milligrams of nitite per liter of water (mg/L) or parts per million (ppm). If you are using an earlier version of the color comparator with one set of concentrations printed on the color strip, you must divide the reading by 3.3 to read the concentration as nitite-nitrogen,.
- Any positive reading (0.15 ppm and above) should be considered potentially toxic to many fishes. If the fishes show signs of stress (increased respiration, clamped fins,etc.) water changes should be made to lower the nitrite concentration to safer levels. Nitrite toxicity can also be reduced in fresh water by the addition of sodium chloride (table salt). A stock solution can be made by adding 1/4 cup of salt to 1 quart of distilled water. Administer at the rate of 1 teaspoon of stock solution per 5 gallons for each 0.5 mg/L of nitrite measured.
CAUTIONS
This kit contains potentially harmful chemicals that can be dangerous if misused. Read label cautions carefully and exercise extreme care during handling, use, and disposal of these chemicals. First aid instructions are available on the label on the reagent package. This kit should not be used by children except under adult supervision. Following use, always clean hands, test apparatus and test area well.
REAGENT AND COLOR STANDARD STABILITY
The reagents should be used before the expiration date shown on the package. If the reagents are to be used after the expiration date they should be checked with an accurate standard solution to insure the reliability of the reagents. The reagents should be stored in a cool place. The color comparator strip should not be exposed to strong light sources for an extended length of time to prevent fading of the colors.
PACKAGING
Kordon's AquaTru Nitrite Test Kits are packaged one kit per box. This kit is available as part of the Kordon AquaTru Aquarist Master Test Kit, Fresh Water Master Test Kit and Saltwater Master Test Kits I & II. Replacement pillows are available in 10 and 50 count bags sufficient for 10 or 50 tests.
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Item: |
Description: |
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35920 |
Nitrite Test Kit |
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35720 |
Nitrite Powder Pillow Reagents- 10 ea. per package |
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34312 |
Nitrite Powder Pillow Reagents- 50 ea. per package |
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2242 Davis Court, Hayward, CA 94545-1114, U.S.A.
Tel. (510) 782-4058 • Fax (510)784-0945 • Toll-Free: (800) 877-7387 • E-mail: info@novalek.com
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